pPT-TAR C1
(Plasmid
#141246)
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PurposeTransformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAGE3.0
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Backbone manufacturerBrumwell et al. 2019
- Backbone size w/o insert (bp) 17531
- Total vector size (bp) 94887
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Modifications to backboneInserted the P. tricornutum mitochondrial genome into the backbone of pAGE3.0
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology ; Diatom Expression
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Selectable markersHIS3 ; Nourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300 (Epicentre)
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Growth instructionsOptional: Arabinose induction to high plasmid copy number.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMitochondrial Genome of Phaeodactylum tricornutum
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Alt nameP. tricornutum
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Alt nameMitochondrial Genome
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SpeciesP. tricornutum
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Insert Size (bp)77356
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MutationMinor additional mutations described in manuscript
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GenBank IDNC_016739.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid is larger than what is usually sequenced at Addgene. See full depositor sequence.
Please visit https://www.biorxiv.org/content/10.1101/2019.12.19.882662v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPT-TAR C1 was a gift from Bogumil Karas (Addgene plasmid # 141246 ; http://n2t.net/addgene:141246 ; RRID:Addgene_141246) -
For your References section:
Rapid method for generating designer algal mitochondrial genomes. Cochrane RR, Brumwell SL, Soltysiak MPM, Hamadache S, Davis JG, Wang J, Tholl SQ, Janakirama P, Edgell DR, Karas BJ. Algal Research, 2020. 50, 102014 10.1016/j.algal.2020.102014
Map uploaded by the depositor.
