Human Improved Genome-wide Knockout CRISPR Library
(Pooled Library #67989)
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Purpose
A gRNA library against 18,010 human genes, designed for use in lentivirus.
gRNAs were redesigned using a new design pipeline as well as an improved scaffold.
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Vector Backbone
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W - does not express Cas9
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 67989 |
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1 | $540 | Add to Cart |
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† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) or with cell lines already expressing Cas9.
Library Details
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SpeciesHuman
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Genes targeted18,010
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gRNAs90,709
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼50 µL
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Concentration20 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Depositor Comments
This library consists of 90,709 guide sequences targeting a total of 18,010 human genes.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:
Human Improved Genome-wide Knockout CRISPR Library v1 was a gift from Kosuke Yusa (Addgene #67989)For your References section:
A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079PubMed 27760321